乳果糖和富氢水对断奶仔猪采食霉变玉米后引起卵巢机能障碍的缓解作用

doi:10.11843/j.issn.0366-6964.2018.12.013

摘要/Abstract

摘要：

旨在研究禾谷镰刀菌属霉变玉米对断奶仔猪卵巢抗氧化水平和NOS的影响以及乳果糖和富氢水的缓解作用。本研究将24头21日龄，平均体重（7.25±1.02）kg的三元杂交断奶仔猪（杜×长×大）随机分为4组（对照组、霉变玉米组、乳果糖缓解组、富氢水缓解组），每组6头。对照组饲喂基础日粮，霉变玉米组、乳果糖缓解组和富氢水缓解组饲喂霉变饲料（呕吐毒素（DON）与玉米赤霉烯酮（ZEA）的含量分别为0.4 mg·kg^-1和0.55 mg·kg^-1）。此外，乳果糖和富氢水缓解组分别用乳果糖（500 mg·kg^-1）和富氢水（10 mL·kg^-1）每天进行两次灌胃。试验期25 d。25 d后屠宰仔猪，收集卵巢，称重；对仔猪卵巢进行组织学观察；检测卵巢组织CAT、GSH-PX、T-SOD、SDH、Caspase-3活性以及NO含量、NOS活性；并用免疫组织化学对nNOS、iNOS和eNOS进行表达定位。结果显示：1）霉变玉米组仔猪相对卵巢重量显著高于对照组（P<0.05），与霉变玉米组相比，乳果糖与富氢水缓解组相对卵巢重量有下降趋势；2）HE结果显示，4组仔猪卵巢均未出现明显病理变化，但霉变玉米组生长卵泡数量显著高于对照组（P<0.05），乳果糖与富氢水缓解组生长卵泡数量与霉变玉米组相比有下降趋势。3）与对照组相比，霉变玉米组CAT活性显著升高（P<0.05），GSH-PX与SDH活性显著下降（P<0.05），T-SOD有下降趋势，Caspase-3活性、NO含量、总NOS活性显著升高（P<0.05）。4）与霉变玉米组相比，乳果糖缓解组GSH-PX与SDH活性显著升高（P<0.05），富氢水缓解组GSH-PX与SDH活性有升高趋势；乳果糖、富氢水缓解组CAT活性有下降趋势，T-SOD活性有上升趋势；乳果糖、富氢水缓解组Caspase-3活性、NO含量显著降低（P<0.05）；乳果糖缓解组总NOS活力有下降趋势，富氢水缓解组NOS活力显著下降（P<0.05）。5）免疫组化结果显示，3种NOS（nNOS、iNOS、eNOS）在各级卵泡的卵母细胞、颗粒细胞、膜细胞均有定位，而且呈细胞特异性表达。霉变玉米中的DON与ZEA可能介导仔猪卵巢组织抗氧化水平降低，从而导致细胞凋亡程度加深；而乳果糖与富氢水通过升高抗氧化水平有效缓解DON与ZEA对仔猪卵巢造成的危害。

Abstract:

The objective of the present study was to investigate the effects of contaminated corn by Fusarium graminearum on the antioxidant levels and NOS of ovary of weaned piglets and the mitigation of lactulose and hydrogen-rich water. Twenty-four weaned piglets of three-hybrid (Duroc×Landrace×Large White) of 21 days old with average body weight (7.25±1.02) kg were randomly divided into 4 groups (control group, contaminated corn group, lactulose relieving group, hydrogen-rich water relieving group), and 6 weaned piglets in each group. The piglets in control group were fed the basal diet, while piglets in contaminated corn, lactulose relieving and hydrogen-rich water relieving groups were fed the moldy feed with the deoxynivalenol (DON) and zearalenone (ZEA) contents of 0.4 mg·kg^-1 and 0.55 mg·kg^-1, respectively. In addition, the lactulose and hydrogen-rich water relieving groups were additionally intragastrically administered twice daily with lactulose (500 mg·kg^-1) and hydrogen-rich water (10 mL·kg^-1), respectively. The experiment lasted for 25 days. After 25 d, piglets were slaughtered, ovaries were collected and weighed. The ovary of piglets was observed histologically, the activities of CAT, GSH-PX, T-SOD, SDH, Caspase-3, NOS and content of NO in ovary were detected. The expressions of nNOS, iNOS and eNOS were also detected by immunohistochemistry. The results showed that:1) The relative ovary weight of the piglets in the contaminated corn group was significantly higher than that in the control group (P<0.05). Compared with the contaminated corn group, the relative ovary weight of piglets in the lactulose relieving group and the hydrogen-rich water relieving group had the downward trend. 2) There were no obvious pathological changes in the ovary of piglets in the 4 groups, but the number of growing follicles in the contaminated corn group were significantly higher than those in the control group (P<0.05). The number of growing follicles in the lactulose and hydrogen-rich water relieving groups decreased(P>0.05) compared with the contaminated corn group. 3) Compared with the control group, the activity of CAT in the contaminated corn group increased significantly, the activities of GSH-PX and SDH decreased significantly (P<0.05) and the T-SOD activity had a decreasing trend, the Caspase-3 activity, NO content, total NOS activity significantly increased (P<0.05). 4) Compared with the contaminated corn group, GSH-PX and SDH activities in the lactulose relieving group significantly increased (P<0.05). GSH-PX and SDH activities in the hydrogen-rich water relieving group had a tendency to increase; CAT activity in lactulose and hydrogen-rich water relieving groups decreased(P>0.05), T-SOD activity increased(P>0.05); Caspase-3 activity and NO content in lactulose and hydrogen-rich water relieving groups decreased significantly (P<0.05). Total NOS activity in lactulose relieving group had a tendency to decrease, while it in hydrogen-rich water relieving group decreased significantly (P<0.05). 5) The results of immunohistochemistry showed that three kinds of NOS (nNOS, iNOS, eNOS) were localized in oocytes, granulosa cells and theca cells of follicles at all stages, and showed cell-specific expression. DON and ZEA in contaminated corn may mediate the decrease of anti-oxidation levels in piglet ovaries, which leads to deeper apoptosis, while lactulose and hydrogen-rich water effectively relieve the harmful effects of DON and ZEA by increasing antioxidant levels in piglet ovaries.

中图分类号:

S828.4

陈亚, 魏全伟, 杜文超, 郑卫江, 丁威, 邢军, 石放雄. 乳果糖和富氢水对断奶仔猪采食霉变玉米后引起卵巢机能障碍的缓解作用[J]. 畜牧兽医学报, 2018, 49(12): 2641-2651.

CHEN Ya, WEI Quan-wei, DU Wen-chao, ZHENG Wei-jiang, DING Wei, XING Jun, SHI Fang-xiong. Mitigation of Lactulose and Hydrogen-rich Water on Ovarian Dysfunction Caused by Dietary Contaminated Corn in Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2641-2651.

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https://www.xmsyxb.com/CN/Y2018/V49/I12/2641

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